IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells

Abstract Background Interferon regulatory factor-1 (IRF1) affects the proliferation of colorectal cancer (CRC). Recombinant interferon inducible protein 35 (IFI35) participates in immune regulation and cell proliferation. The aim study was to examine whether IRF1 radiation sensitivity CRC by regulating IFI35. Methods CCL244 SW480 cells were divided into five groups: blank control, IFI35 upregulation, upregulation downregulation, downregulation control. All groups treated with X-rays (6 Gy). activation detected luciferase reporter assay. GEPIA database used CRC. characterized using CCK-8, EdU, cycle, clone formation, flow cytometry, reactive oxygen species (ROS), mitochondrial membrane potential. Nude mouse animal models detect effect on Results can bind promoter promote expression consistency CRC, according (R = 0.68, p < 0.0001). After irradiation, inhibited colony formation promoted apoptosis ROS, while reduced apoptosis, potential also reduced. vivo experiments supported vitro ones, smaller tumors fewer liver metastases upregulation. Conclusions cells. is involved radiosensitivity might be a target for radiosensitization.